List of Articles by MeSH

EXTRACELLULAR MATRIX

1. Reichert C, Klein M, Kasaj A, Kuhn S, Götz H, Hommel G, Duschner H, Al-Nawas B. Modulation of Osteogenic Cell Morphology by ECM Ligands and Enamel Matrix Derivative. Acta Stomatol Croat. 2009;43(3):188-201.
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   Title in English:	Modulation of Osteogenic Cell Morphology by ECM Ligands and Enamel Matrix Derivative
   Title in Croatian:	Modulacija morfologije osteogenih stanica pomoću ECM liganada i derivata caklinskog matriksa
   Type of Article:	original scientific paper
   MeSH:	OSTEOBLASTS EXTRACELLULAR MATRIX ENAMEL MATRIX PROTEINS CYTOSKELETON PERIODONTAL DISEASES
   Abstract:	The precondition for successful periodontal regeneration is adequate activation of relevant cell populations like osteogenic cells. Here, cell adhesion and maturation are closely associated with cell morphology and f-actin cytoskeletal organisation. The potential of solitaire extracellular matrix (ECM) components as well as enamel matrix derivative (EMD) to enhance periodontal healing is well documented. Objective: The aim of the study was to test the impact of the ECM proteins collagen type 1 and laminin-1 as well as commercially available EMD on osteogenic cell morphology and cytoskeletal organisation. Material and methods: In an observational study, a total of 2450 osteogenic cells of 5 different cell lines (4 primary ones and 1 commercial one) cultivated on the respective substrates were analysed by 3 independent observers. After staining for the f-actin cytoskeleton and automated CLSM visualisation, cells were assigned to 3 different categories depending on morphological cell attributes (immature vs. intermediate vs. mature). Besides descriptive analysis, a multivariate logistic regression was performed to identify relevant influence parameters on cell morphology and cytoskeletal organisation. Results: The applied solitaire ligands collagen and laminin and especially EMD promoted a mature osteogenic phenotype. Nevertheless, considerable differences between the investigated cell lines could be identified as well. Analysis of cell morphology and cytoskeletal organisation offers a reliable method of acquiring the first hints of biocompatibility and bio-activation on different substrates. Conclusion: Our results highlight the potential of the investigated ligands to support periodontal regeneration by enhancing osteogenic cell attachment and maturation.