List of Articles by MeSH

ENTEROCOCCUS FAECALIS

1. Brugger W, Hofer V, Städtler P. Antibacterial Effects of Endodontic Dressings on Enterococcus Faecalis in Human Root Dentine. Acta Stomatol Croat. 2007;41(4):326-36.
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   Title in English:	Antibacterial Effects of Endodontic Dressings on Enterococcus Faecalis in Human Root Dentine
   Title in Croatian:	Antibakterijski učinak endodontskog uloška na Enterococcus faecalis u dentinu zubnog korijena
   Type of Article:	original scientific paper
   MeSH:	ENDODONTICS ENTEROCOCCUS FAECALIS DENTINE, SECONDARY ANTI-INFECTIVE AGENTS, LOCAL
   Abstract:	The aim of this in vitro study was to assess antimicrobial activity of calcium hydroxide (Calasept) and chlorhexidine (CHX) in various concentrations with respect to Enterococcus faecalis in human root dentine up to 100 μm. Material and Methods: Forty-eight human root canals were enlarged to standard size (ISO 40) and inoculated with Enterococcus faecalis for 21 days. After inoculation the canals were medicated with one of the following: 2 %, 1 % and 0.2 % chlorhexidin gel, Chlorhexidine releasing gutta-percha points (active point), calcium hydroxide and aqua distillate (aqua dest.). Aqua dest. served as control medium. At the end of a disinfection period of one week dentine samples were collected with reamer and H-file (ISO 45 and 50) and put onto Columbia agar plates. Results: Bacterial growth was assessed by counting the colony forming units (CFU) after 24 hours and 48 hours of incubation. CHX gels could penetrate dentine up to 100 μm. Two % CHX gel is slightly stronger than 1 % CHX gel (p-value 0.0925) only in the peripheral dentine sample after 48 hours of incubation, and 0.2 % CHX gel had less effects on E. faecalis than 2 % CHX (p-value 0.0191). In the central dentine sample no difference between the CHX gels could be observed. In general, CHX gels were more effective than the other medicaments tested. No significant difference between Ca(OH)2 and Aqua dest. could be observed. Conclusion: For effective elimination of E. faecalis, especially in deeper dentine-layers, CHX concentrations of at least 1 % are necessary. An intracanal, interappointment dressing of 2 % CHX may have the potential to eliminate E. faecalis in vivo.

2. Vrček D, Prpić-Mehičić G, Tambić-Andrašević A, Poljak-Guberina R, Galić N, Katunarić M. The Antimicrobial Effect of Calasept, Superlux Calcium Hydroxide Liner and Gutta-percha with Calcium Hydroxide. Acta Stomatol Croat. 2002;36(2):203-12.
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   Title in English:	The Antimicrobial Effect of Calasept, Superlux Calcium Hydroxide Liner and Gutta-percha with Calcium Hydroxide
   Title in Croatian:	Antimikrobni učinak Calasepta, Superlix calcium hydroxid i gutaperka štapića presvučenih kalcijevim hidroksidom
   Type of Article:	original scientific paper
   MeSH:	CALCIUM HYDROXIDE ENTEROCOCCUS FAECALIS CANDIDA ALBICANS GUTTA-PERCHA
   Abstract:	Microorganisms are the main cause of disease in root canals and periapically. Facultative bacteria and fungi have been identified in persistent endodontic infection. The objective of this study was to determine in vitro the efficacy of: Calasept (Speiko, Darmstadt, Germany), Superlux calcium hydroxide liner (Dental Material Gesellschaft, Hamburg, Germany) and gutta-percha with calcium hydroxide (Roeko, Langenau, Germany) in direct contact with microorganisms. The microorganisms used were: Enterococcus faecalis (ATCC 29212) and Candida albicans (NCTC 3123). An overnight mixed broth culture of microorganisms (108 CFU/ml) was prepared. Paper points and gutta-percha points were immersed in suspension for 5 min. The paper points were then covered with Calasept and Superlux calcium hydroxide liner. At intervals of 0; 6; 12; 24; 48 and 72 h at 37°C, aerobically. The antimicrobial effect of Calasept and Superlux calcium hydroxide liner apparently occured after 6h on Enterococcus faecalis and 12 h on Candida albicans. With gutta-percha, this effect did not occur for either Enterococcus faecalis or Candida albicans after 72 h.

3. Jukić S, Vidučić D, Miletić I, Božić Ž, Kalenić S, Anić I. Antibacterial Eeffect of Er: YAG Laser in the Root Canal. Acta Stomatol Croat. 2001;35(2):195-204.
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   Title in English:	Antibacterial Eeffect of Er: YAG Laser in the Root Canal
   Title in Croatian:	Antibakterijski učinak Er:YAG lasera u korijenskom kanalu
   Type of Article:	original scientific paper
   MeSH:	LASERS STAPHYLOCOCCUS AUREUS ENTEROCOCCUS FAECALIS ROOT CANAL THERAPY ANTIBACTERIAL AGENTS
   Abstract:	The aim of this study was to evaluate the antibacterial effect of Er:YAG laser in the root canal using bacteria of Enterococcus and Staphylococcus species. Eleven single-rooted teeth were instrumented, sterilized and ten of them inoculated with bacterial suspension of concentration 106 CFU/ml in brain heart broth. Nine samples were irradiated with Er:YAG laser (220 mJ/40 Hz/10 s) in two cycles with a pause of 15 s for cooling. One sample with bacterial suspension without irradiation was a positive control, and one sterile sample a negative control. After irradiation the samples were placed in vials with broth and the number of CFU/ml was determined by bacterial analysis after one and two weeks. Er:YAG laser did not completely sterilize any canal, but in three cases elimination of Staphylococcus aureus occurred. This indicates the possible better antibacterial effects of erbium laser in the root canal if appropriate energy delivery system could be applied.

4. Karlović Z, Anić I, Miletić I, Prpić-Mehičić G, Pezelj-Ribarić S, Maršan T. Antibacterial activity of halothane, eucalyptol and orange oil. Acta Stomatol Croat. 2000;34(3):303-9.
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   Title in English:	Antibacterial activity of halothane, eucalyptol and orange oil
   Title in Croatian:	Antibakterijsko djelovanje halotana, eukaliptola i narančina ulja
   Type of Article:	original scientific paper
   MeSH:	HALOTHANE MENTHOL OILS + pharmacology STAPHYLOCOCCUS AUREUS ENTEROCOCCUS FAECALIS FRUITS ANTI-INFECTIVE AGENTS, LOCAL
   Abstract:	Staphylococcus aureus and Enterococcus faecalis species were used for the experiment. 0,5 McFarland bacterial inoculum was diluted using saline in proportions 1:10, 1:10 2, 1:10 3, 1:10 4, 1:10 5 and 1:10 6. 0.2 millilitre of solving agent was mixed with an equal quantity of bacterial suspension. Sixty specimens, 10 for each proportion, were prepared. Specimens were hermetically closed and left for 10 and 30 minutes. 0.1 millilitre of each specimen was planted to the blood agar and put in the thermostat at 37°C for 24 hours. The procedure was repeated twice. Halothane exhibited greatest antibacterial activity, destroying all concentrations of Staphylococcus aureus and Enterococcus faecalis. Eucalyptol showed activity towards Staphylococcus aureus, while orange oil did not show any antibacterial effect on the examined bacteria.

5. Pavelić B, Anić I, Jukić S, Stilinović B, Najžar-Fleger D. [Antimicrobial effect of calcium hydroxide on S.mutans, S.faecalis and C.albicans in vitro in liquid media]. Acta Stomatol Croat. 1995;29(4):219-24.
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   Title in English:	Antimicrobial effect of calcium hydroxide on S.mutans, S.faecalis and C.albicans in vitro in liquid media
   Title in Croatian:	Antimikrobni učinak kalcijeva hidroksida na streptococcus mutans, streptococcus faecalis i candida albicans, in vitro, u tekućem hranilištu
   Type of Article:	original scientific paper
   MeSH:	CALCIUM HYDROXIDE STREPTOCOCCUS MUTANS + drug effects ENTEROCOCCUS FAECALIS CANDIDA ALBICANS MICROBIAL SENSITIVITY TESTS CULTURE MEDIA
   Abstract:	(not available)

6. Pavelić B, Anić I, Najžar-Fleger D, Stilinović B, Temmer K. [The antimicrobial efficiency of aqueous solutions of calcium hydroxide on Streptococcus mutans, Streptococcus faecalis and Candida albicans, in vitro]. Acta Stomatol Croat. 1991;25(4):207-12.
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   Title in English:	The antimicrobial efficiency of aqueous solutions of calcium hydroxide on Streptococcus mutans, Streptococcus faecalis and Candida albicans, in vitro
   Title in Croatian:	Antimikrobni učinak vodenih pripravaka kalcijevog hidroksida na Streptococcus mutans, Streptococcus faecalis i Candida albicans, in vitro
   Type of Article:	original scientific paper
   MeSH:	STREPTOCOCCUS MUTANS + drug effects CANDIDA ALBICANS ENTEROCOCCUS FAECALIS CALCIUM HYDROXIDE MICROBIAL SENSITIVITY TESTS
   Abstract:	Calcium hydroxide preparations Ca(OH)2 are used as water solutions, putly, liner, cement or artificial material. The therapeutic effect of each medicaments depends on physical and chemical characteristic of additional components. The aim of the paper is to research the sensitivity of Streptococcus faecalis and Candida albicans to different water solution concentrations of calcium hydroxide. As solid plates, Bacto-Mitis-Salivarius agar was used for S. mutans, Torlak blood agar for S. faecalis and Sabourand agar "Torlak" for C. albicans. The holes were drilled in solid plates and were filled by 0.2 ml of water solutions of Ca(OH)2. Preparations, created as described were incubated aerobically at 37 degrees C in the period of 48 hrs. Results were read after 24 as well as after 48 hrs. The examined solutions of Ca(OH)2, revealed the highest antimicrobic effect after 24 hrs. The result remained unchanged even after 48 hrs. The inhibition of S. mutans growth on the solid plate occurred with the concentration of Ca(OH)2 solution from 271.18 x 10(-2) mol/dm3 and pH 12.76. The growth of S. faecalis at the solid plate was inhibited with the concentration of 3335.21 x 10(-3) mol/dm3 and pH 12.82. The inhibition of C. albicans growth on solid plate occurred with the concentration of Ca(OH)2 solution from 1023.20 x 10(-3) mol/dm3 and pH 12.84. The difference in the sensitivity of the examined microorganisms at the same concentrations of Ca(OH)2 solution was observed.