List of Articles by Author
- Presečki Ž, Brkić H, Primorac D, Drmić I. Methods of preparing the tooth for DNA isolation. Acta Stomatol Croat. 2000;34(1):15-24.
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Full Text
| Title in English: |
Methods of preparing the tooth for DNA isolation |
| Title in Croatian: |
Postupci pripreme zuba za izolaciju deoksiribonukleinske kiseline (DNK) |
| Type of Article: |
preliminary report |
| MeSH: |
DNA FORENSIC DENTISTRY + methods DENTAL PULP DNA, MITOCHONDRIAL PHENOLS CHLOROFORM |
| Abstract: |
In this paper, the importance of the tooth in identification an unknown person has been demonstrated.The theoretical part shows the difference between genomic and mitochondrial DNA. That difference is the durability and properties which they carry and the value they have in identification. Microanatomy has also been described, possible locations of cells inthe tooth and methods to collect material for DNA extraction.The practical part shows the methods used in particular cases for DNA isolation. Horizontal section through the cervical root is used to collect material for DNA isolation. Genomic DNA , from the material, is extracted by organic phenol-chloroform methods. The results are shown on 1 % agarose gel and photographed by a Polaroid camera. |
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