List of Articles by Author

NOTES:

*This author has been accepted with these names:

Bešlić, Snježana
Bešlić, S

*Articles are cited according to Uniform Requirements by International Committee of Medical Journal Editors - ICMJE and NLM Style Guide for Authors, Editors and Publishers

Kovačević M, Šnjarić D, Horvat J, Tamarut T, Kuiš D, Bešlić S. Methods for Histological Evaluation of the Deposition of Newly Created Bone after Endodontic Therapy in the Dog - a Preliminary Report. Acta Stomatol Croat. 2004;38(3):201-13.

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Šnjarić D, Kovačević M, Bešlić S, Tamarut T, Horvat J, Kuiš D. Doxycycline influence on bone remodeling after therapy of periapical lesions. Acta Stomatol Croat. 2003;37(3):376-7.

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Title in English:	Doxycycline influence on bone remodeling after therapy of periapical lesions
Title in Croatian:	Utjecaj doksiciklina na preoblikovanje kosti nakon terapije periapeksne lezije
Type of Article:	congress abstract
MeSH:	PERIAPICAL DISEASES + therapy DOXYCYCLINE BONE REMODELING
Abstract:	The aim of the study was to evaluate bone remodelling after endodontic therapy of periapical lesions combined with doxycycline administration by measuring osteoid surface, osteoid thickness, osteoclast index and inflammatory cell count in comparison with bone remodelling after endodontic therapy whithout antibiotic administration in dogs.The experiment was conducted on six mongrel dogs. Endodontic therapy was performed 35 days after artificial dental pulp exposure. A total of 30 roots were cleaned and shaped by Crown-down technique with Profile® rotary instruments and filled with Thermafill® at the lenght to the apical delta confirmed radiographically. Cavities were sealed with amalgam. Animals were then divided into group 1 (antibiotic, three animals with 21 roots) and group 2 (non-antibiotic, three animals with 17 roots). Immediately after endodontic therapy, animals in group 1 were treated by administration of doxycycline (10 mg/kg, Hiramicin, Pliva d.d., Zagreb, Croatia) for a period od12 days. All animals were sacrified 35 days after endodontic therapy and mandibles embedded in methylmetacrylate. Undemineralized sections 5 µm thick were stained with toluidine blue. The histomorphometric indices (osteoid surface, osteoid thickness, osteoclast index and inflammatory cell count) were measured by light microscopy using computer program (ISSA, Vams, Zagreb,Croatia).Osteoid thickness (group 1: 15.33 µm ± 33.49; group 2: 15.62 µm ± 7.41 ; p > 0.05) and inflammatory cell count (group 1: 117.16 ± 38.66; group 2: 111.39 ± 75.81; p > 0.05) did not show statistically significant difference. The difference between groups was statistically significant in measured indices of osteoid surface and osteoclast index.Osteoid surface was greater in group 1 (group 1: 30.56 ± 15.51; group 2: 10.34% ± 11.60; p < 0.0001). Osteoclast index was lower in group 1 (group 1: 43.13 mm - 2 ± 41.25; group 2: 111.34 mm - 2 ± 115.46 ; p < 0.0001). Statistical analysis was performed using Mann-Whitney U test.There was no difference in osteoid thickness and inflammatory cell count between the two groups. Osteoid surface was higher and osteoclast index lower in group 1 (antibiotic treated group) which indicates greater bone tissue healing potential.

Kuiš D, Kovačević M, Tamarut T, Bešlić S, Šnjarić D, Horvat J. Evaluation of Bone Remodelling After Endodontic Therapy of the Periapecal Lesion. Acta Stomatol Croat. 2003;37(3):355-6.

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Title in English:	Evaluation of Bone Remodelling After Endodontic Therapy of the Periapecal Lesion
Title in Croatian:	Ocjena preoblikovanja kosti nakon endodontske terapije periapeksne lezije
Type of Article:	congress abstract
MeSH:	ROOT CANAL THERAPY + adverse effects PERIAPICAL DISEASES + complications
Abstract:	The aim of the study was to evaluate bone remodelling by measuring osteoid surface, osteoid thickness, osteoclast index and inflammatory cell count after two differnet approaches in endodontic therapy of periapecal lesion on dogs’ teeth.After inducing periapecal lesion in 23 mandibular premolars (37 root canals) from six mongrel dogs, 17 root canals were instrumented to the apical delta with crowndown techniwue using ProFile® Ni-Ti rotary instruments and filled to the same length with Thermafill® obturation technique and Top Seal® cement (group 1). The other 20 root canals (group 2) were instrumented using controlled overinstrumentation technique to the point determined ellectronically (electronic apex locator EED-11, Struja, Zagreb, Croatia). Teeth were obturated with Thermafill® at the length 2mm shorter than the working length. Length of obturation was confirmed radiographically and access cavities sealed with amalgam in both groups. The animals were sacrified 35 days after the end of endodontic treatment. Undemineralizad sections 5-7 mm thick were stained with toluidine blue. The histomorphometric indices (oseoid surface, osteoid thickness, osteoclast index and inflammatory cell count) were measured by light microscopy using computer program (ISSA, Vams, Zagreb, Croatia).The difference between grops was statistically significant in all measured indices. In group 1 the osteoid surface was lower (10.34 % ± 11.60) as well as osteiod thickness (15.62 µm ± 7.41) and inflammatory cell count (111.39 ± 75.81) while osteoclast index was higher (111.34 mm - 2 ± 115.46). In group 2 osteoid surface was higher (33.21 % ± 21.43) as well as osteoid thickness (16.26 µm ± 6.46) and inflammatory cell count (137.62 ± 46.34), while osteoclast index was lower (27.00 mm - 2 ± 39.03). Stastistical analysis was performed using Menn-Whitney U Test.Results obtained by measuring histomorphometric indices indicate sustained bone resorption activity in group 1 anf faster bone formation in group 2. The greater number of inflammatory cells in group 2 was probaly caused by additional trauma of periapical tissues during overinstrumentation. This trauma does not interfere with bone remodelling.

Kovačević M, Tamarut T, Bešlić S, Zoričić S, Bobinac D. Histological Correlation Between Pulp and Periapical Tissue Findings. Acta Stomatol Croat. 2003;37(3):351-2.

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Title in English:	Histological Correlation Between Pulp and Periapical Tissue Findings
Title in Croatian:	Korelacija histopatološkog nalaza pulpnog i periapeksnog tkiva
Type of Article:	congress abstract
MeSH:	DENTAL PULP CAVITY + anatomy & histology PERIAPICAL TISSUE
Abstract:	The aim of the study was to analyse the correlation between histopathological findings of pulp and periapical tissue during open infection of the pulp on the experimental dog model.Nine mongrel dogs were divided into 4 experimental groups. Pulps of mandibular premolars (37 roots) were exposed using low-speed dental engine. Teeth were left open to the oral environment for 20 days (group I - two animals with eight roots), 35 days (group II - three animals with 15 roots), 50 days (group III - two animals with 6 roots) ahd 65 days (group IV - two animals with eight roots) to induce pulpitis and apical parodontitis. Pulp of the mesial root (nine roots) of a mandibular molar (control tooth) were not exposed to the oral environment. After the experimental period animals were sacrificed. Undemineralized teeth with bone, embedded in methylmetacrylate, were prepared for histological analysis (toluidine blue).After 20 days exposure (group I9 six roots had partial pulp necrosis and two roots complete pulp necrosis. In group II (35 days exposure) three pulps had pulpitis, six roots had complete pulp necrosis and six partial pulp necrosis. In group III (50 days exposure) two roots had pulpitis, three partial pulp necrosis and one complete pulp necrosis. In group IV (65 days) all pulps (eight) had complete pulp necrosis. All teeth had pulpitis (five roots), regardless of the experimental period, had acute serose apical periodontitis in periapical tissue, all teeth (15 roots) with partial necrosis had subacute apical periodontitis in the periapical tissue. Teeth with complete pulp necrosis (19 roots) had chronic apical periodontitis and in one case in group III abscessus periapicalis. Correlation between histopathological findings of the pulp and periapical tissue is statistically significant (Pearson correlation; r = 0.92, p < 0.01).The condition of the pulp correlated with the histopathological findings of periapical tissue in the open types of the pulp infection. Acute apical periodontitis with bone resorption began during pulpitits and can occur before 20 days of the pulp exposure in the dog.

Horvat J, Kovačević M, Bešlić S, Kuiš D, Šnjarić D. Patterns of Osteoid Deposition in Periapical Lesion Healing Using Vital Dye. Acta Stomatol Croat. 2003;37(3):331-2.

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Title in English:	Patterns of Osteoid Deposition in Periapical Lesion Healing Using Vital Dye
Title in Croatian:	Odlaganje osteoida prilikom cijeljenja periapeksne lezije prikazano vitalnom bojom
Type of Article:	congress abstract
MeSH:	PERIAPICAL DISEASES + therapy
Abstract:	The aim of the study was to describe qualitively and quantitively osteoid deposition in periapecal lesion healing on dogs’ teeth after endodontic therapy based on two different approaches.Periapical lesions were experimentally induced in six mongrel dogs by exposing the pulps to the oral enviornment for 35 days. Animals were randomly divided into two groups. Seventeen root canals were instrumented to the apical delta with crown-down technique using ProFile® Ni-Ti rotary instruments and filled with Thermafill® guttapercha and Topseal® cement (group 1). In group 2, 20 root canals were instrumented using controlled overinstrumentation technique to the point determined using eletronic apex locator EED 11 (Struja, Zagreb, Croatia). Teeth were obturated with Thermafill® at the length 2 mm shorter than the working length, and access cavities sealed with amalgam The length of obturation was confirmed radiographically in both groups. On the same day, one animal in each group was injected intraperitoneally with vital dye Procion Brilliant Red H8-BS (ICI Organics, Providence, R.I., USA). Animals were sacrified 35 days after the end of endodontic tretment. Undemineralized and unstained sections 5-7 µm thick were first viewed under UV light for fluorescence. The histomorphometric indices (osteoid surface and thickness) were measured by light microscope using computer program ISSA (Vams, Zagreb, Croatia) on Toluidine Blue stained sections and compared with qualitative analysis of vital dye staining.Results Under UV light vital dye showed predominantly fluorescent lines marking the border of newly formed bone. Those lines were more numerous. Brighter and further away from resorbed bone surface in group 2. Fine fluorescent trabeculae and double rings around Haversian canals were seen in group 2 only. Osteoid thickness was significantly greater in group 2 (group 1: 15.62 µm ± 7.41; group 2: 16.26 µm ± 6.46; p < 0.001) as well as oteoid surface (group 1:10.34 % ± 11.60; group 2: 33.21 % ± 21.43; p < 0.001). Statistical analysis was performed using Mann-Whitney U test. Conclusion Numerous areas of fluorescence and greater osteoid thickness and osteiod surface show greater bone matrix preduction in group 2.

Kovačević M, Tamarut T, Zoričić S, Bešlić S. A Method for Histological, Enzyme Histochemocal and Immunohistochemical Analysis of Periapical Diseases on Undecalcified Bone with Teeth. Acta Stomatol Croat. 2003;37(3):261-73.

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