List of Articles by Author

1. Kovačević M, Tamarut T, Bešlić S, Zoričić S, Bobinac D. Histological Correlation Between Pulp and Periapical Tissue Findings. Acta Stomatol Croat. 2003;37(3):351-2.
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   Title in English:	Histological Correlation Between Pulp and Periapical Tissue Findings
   Title in Croatian:	Korelacija histopatološkog nalaza pulpnog i periapeksnog tkiva
   Type of Article:	congress abstract
   MeSH:	DENTAL PULP CAVITY + anatomy & histology PERIAPICAL TISSUE
   Abstract:	The aim of the study was to analyse the correlation between histopathological findings of pulp and periapical tissue during open infection of the pulp on the experimental dog model.Nine mongrel dogs were divided into 4 experimental groups. Pulps of mandibular premolars (37 roots) were exposed using low-speed dental engine. Teeth were left open to the oral environment for 20 days (group I - two animals with eight roots), 35 days (group II - three animals with 15 roots), 50 days (group III - two animals with 6 roots) ahd 65 days (group IV - two animals with eight roots) to induce pulpitis and apical parodontitis. Pulp of the mesial root (nine roots) of a mandibular molar (control tooth) were not exposed to the oral environment. After the experimental period animals were sacrificed. Undemineralized teeth with bone, embedded in methylmetacrylate, were prepared for histological analysis (toluidine blue).After 20 days exposure (group I9 six roots had partial pulp necrosis and two roots complete pulp necrosis. In group II (35 days exposure) three pulps had pulpitis, six roots had complete pulp necrosis and six partial pulp necrosis. In group III (50 days exposure) two roots had pulpitis, three partial pulp necrosis and one complete pulp necrosis. In group IV (65 days) all pulps (eight) had complete pulp necrosis. All teeth had pulpitis (five roots), regardless of the experimental period, had acute serose apical periodontitis in periapical tissue, all teeth (15 roots) with partial necrosis had subacute apical periodontitis in the periapical tissue. Teeth with complete pulp necrosis (19 roots) had chronic apical periodontitis and in one case in group III abscessus periapicalis. Correlation between histopathological findings of the pulp and periapical tissue is statistically significant (Pearson correlation; r = 0.92, p < 0.01).The condition of the pulp correlated with the histopathological findings of periapical tissue in the open types of the pulp infection. Acute apical periodontitis with bone resorption began during pulpitits and can occur before 20 days of the pulp exposure in the dog.

2. Kovačević M, Tamarut T, Zoričić S, Bešlić S. A Method for Histological, Enzyme Histochemocal and Immunohistochemical Analysis of Periapical Diseases on Undecalcified Bone with Teeth. Acta Stomatol Croat. 2003;37(3):261-73.
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   Title in English:	A Method for Histological, Enzyme Histochemocal and Immunohistochemical Analysis of Periapical Diseases on Undecalcified Bone with Teeth
   Title in Croatian:	Postupak histološke, histokemijske i imunohistokemijske raščlambe periapikalnih bolesti na nedekalcificiranoj kosti sa zubima
   Type of Article:	original scientific paper
   MeSH:	PERIAPICAL DISEASES + immunology
   Abstract:	The objective was to examine and apply a method for serial sectioning of undecalcified canine teeth with surrounding jawbone and to analyse it histologically, enzyme histochemically and immunohistochemically. Pulpitis and apical periodontitis were induced in a mongrel dog’s premolar teeth by exposing the pulp to the oral environmental influence for 50 days. After animal sacrifice undecalcified bone with the decoronated experimental teeth were embedded in methylmethacrylate and sectioned with a tungsten carbide knife at 5-7 μm. Sections were stained with toluidin blue (TB) for histological analysis and by a method of staining non-specific acid phosphatase (ACP), and tartrat-resistant acid phosphatase (TRAP). Immunohistochemical staining was performed for detection of CD 45+ lymphocytes. Because the specimens were 5-7 μm thick it was possible to perform a detailed cytological analysis of changes in the pulp and periapex in situ with a light microscope on histological and enzyme histochemical specimens. Immunohistochemical staining was not satisfactory. The method of embedding undecalcified bone and teeth in methylmethacrylate and sectioning with a tungstencarbide knife is satisfactory for histological and some enzyme histochemical analysis in endodontic research. However, immunohistochemical staining needs improvement.